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Identification of NSUN2 knockout efficiency at the genome level and transcriptome level. A. NSUN2 knockout characteristics were identified by Sanger sequencing in the QGY-7703 cell genome. B. The mRNA expression levels of NSUN2 in NSUN2 knockout cells (KO6/KO10) and NSUN2 reconstituted cells were evaluated by real-time PCR. WT, wilg type ; KO, knock out ; Res, rescue.
