Figure 1.

Time-series dataof paired expression and chromatin accessibilityreveal the regulatory landscape for rumen development

A. Experimental design diagram for multi-replicate, multi-tissue, and multi-level omics data profiling during sheep development from E60 to postnatal stages (D1, D7, and D28) to Y1. Hierarchical clustering of gene expression for 14,637 genes (B) and chromatin accessibility for 178,651 OCRs (C). Unsupervised PCA of rumen gene expression (D) and chromatin accessibility (E). ATAC-seq, Assay for Transposase-Accessible Chromatin with high-throughput sequencing; RNA-seq, RNA sequencing; E60, embryonic day 60; D1, postnatal day 1; D7, postnatal day 7; D28, postnatal day 28; Y1, adult 1 year; OCR, open chromatin region; PCA, principal component analysis; PC, principal component.