Figure 4.

18 rumen TTFs and their upstream regulations

A. Phylogeny of 50 tissues from sheep based on the expression of 18 rumen TTFs groups the samples well by different lineages and biological systems. B. Biological functions of 18 rumen TTFs (marked in green) and the tissue with high expression (marked in blue). Tissues are grouped and colored by their lineages. C. 18 rumen TTFs are grouped into early-development (cold-colored) and late-development (warm-colored) by their dynamic expression patterns during the developmental stages. In addition, 18 rumen TTFs associated with specific cell type names were visualized by a uniform manifold approximation and projection plot in skin organoid scRNA-seq data. D. The upstream gene regulatory network of rumen TTFs shows the candidate TFs with statistical significance. Nodes are colored by early- and late-development TTFs. Blue edges highlight the regulatory relationship among TTFs.