Table 2.
VP1:2:3 ratio of Sf9-rAAV and HEK-rAAV
VP | Apparent MW (Da) | Sf9 |
HEK293 |
||||
---|---|---|---|---|---|---|---|
Run 1 | Run 2 | Average | Run 1 | Run 2 | Average | ||
VP1 | 87 | 7.2 | 7.2 | 7.2 | 14.7 | 15.4 | 15.1 |
VP2 | 72 | 19.2 | 15.9 | 17.6 | 16.2 | 17.2 | 16.7 |
VP3 | 62 | 73.7 | 51.3 | 62.5 | 60.2 | 44.3 | 52.3 |
The VP1:2:3 ratios of HEK-rAAV (10 L) and Sf9-rAAV (2 L) were calculated based on the signal strength from Coomassie blue staining gel images.