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. 2023 Nov 20;32(1):74–83. doi: 10.1016/j.ymthe.2023.11.022

Table 3.

The genomic DNA profile of Sf9-rAAV and HEK-rAAV was assessed by PacBio

DNA source Sf9-rAAV HEK-rAAV
AAV vector genome 99.29% 98.84%
Rep-Cap plasmid 0.06% 0.40%
GOI plasmid backbone and pHelper plasmid or baculoviral sequence 0.62% 0.34%
HCD 0.03% 0.38%
Chimeric events with HCD 0.00% 0.04%

Sf9-rAAV (2 L) and HEK-rAAV (50 L) were tested by PacBio NGS. The percentage was calculated by dividing the reads of each using the total unique mapped reads.