Table 3.
The genomic DNA profile of Sf9-rAAV and HEK-rAAV was assessed by PacBio
| DNA source | Sf9-rAAV | HEK-rAAV |
|---|---|---|
| AAV vector genome | 99.29% | 98.84% |
| Rep-Cap plasmid | 0.06% | 0.40% |
| GOI plasmid backbone and pHelper plasmid or baculoviral sequence | 0.62% | 0.34% |
| HCD | 0.03% | 0.38% |
| Chimeric events with HCD | 0.00% | 0.04% |
Sf9-rAAV (2 L) and HEK-rAAV (50 L) were tested by PacBio NGS. The percentage was calculated by dividing the reads of each using the total unique mapped reads.