FIG. 3.

ELISPOT estimates of the frequencies of cells that produced IL-4 or IFN-γ in response to hsp65 among splenocytes from hsp65 DNA-immunized or M. tuberculosis-infected mice. Purified CD4⁺/CD8⁻ and CD8⁺/CD4⁻ cells were amplified by culture for 14 days on J774-hsp65 cells before assay in the presence of J774-hsp65 cells. The results shown are mean estimates (± SD) from triplicate wells at 3 different dilutions after subtraction of estimates obtained by assay in the presence of J774 vector cells (about 10 per 1,000 for CD4⁺/CD8⁻ cells and 12 per 1,000 for CD8⁺/CD4⁻ cells).