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Association of IFN-γ production with CD44hi cells. Purified CD4+/CD8− and CD8+/CD4− cells were amplified by culture for 14 days on J774-hsp65 cells and then separated into CD44hi and CD44lo by FACSort and amplified for a further 12 days before assay in the presence of J774-hsp65 cells as described in the legend to Fig. 3.
