REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-MCL-1	Rockland Immunochemicals	Cat# 600-401-394S, RRID:AB_11179937
Rabbit monoclonal anti-actin (HRP)	Cell Signaling Technology	Cat #5125, RRID: AB_1903890
Mouse monoclonal phospho-H2AX S139	Millipore	Cat# 05–636, RRID:AB_309864
Rabbit polyclonal anti-phospho-CHK1 S345	Abcam	Cat# ab58567, RRID:AB_10563825
Rabbit polyclonal anti-phospho-RPA32 S33	Bethyl	Cat# A300-246A, RRID:AB_2180847
Goat Anti-rabbit AlexaFluor® 488 secondary antibody	Thermo Fisher Scientific	Cat# A-11034, RRID:AB_2576217
Goat Anti-mouse AlexaFluor® 488 secondary antibody	Thermo Fisher Scientific	Cat# A-11001, RRID:AB_2534069
Goat Anti-rabbit AlexaFluor® 568 secondary antibody	Thermo Fisher Scientific	Cat# A-11011, RRID:AB_143157
Goat Anti-rabbit AlexaFluor® 594 secondary antibody	Thermo Fisher Scientific	Cat# A-11012, RRID:AB_2534079
Mouse monoclonal anti-MCL-1	Proteintech	Cat# 66026-1-Ig, RRID:AB_11041711
Mouse monoclonal anti-FLAG	Sigma-Aldrich	Cat# A8592, RRID:AB_439702
Rabbit monoclonal anti-phospho-histone H3 S10	Cell Signaling Technology	Cat# 53348, RRID:AB_2799431
Mouse monoclonal anti-MCM5	Santa Cruz Biotechnology	Cat# sc-165994, RRID:AB_2142526
Rabbit polyclonal anti-MCM5	Bethyl	Cat# A300-195A, RRID:AB_185552
Chemicals, peptides, and recombinant proteins
Complete protease inhibitor cocktail tablets	Sigma-Aldrich	Cat# 05056489001
Propidium iodide	Thermo Fisher Scientific	Cat# P3566
DAPI	Cell Signaling Technology	Cat# 4083
MitoTrackerTM Deep Red FM	Thermo Fisher Scientific	Cat# M22426
EPPS	Thermo Fisher Scientific	Cat# J61296.AE
Trypsin	Promega	Cat# VA9000
LysC	Promega	Cat# VA1170
TMTpro™ 16plex Label Reagent Set	Thermo Fisher Scientific	Cat# A44522
FxCycleViolet	Thermo Fisher Scientific	Cat# R37166
Lipofectamine™ LTX Reagent with PLUS™ Reagent	Thermo Fisher Scientific	Cat# 15338100
S63845	Selleck Chemicals	Cat# S8383
ABT-199	Selleck Chemicals	Cat# S8048
Vincristine	Selleck Chemicals	Cat# S9555
Nocodazole	Selleck Chemicals	Cat# S2775
Vinorelbine	Selleck Chemicals	Cat# S4269
Paclitaxel	Selleck Chemicals	Cat# S1150
Combretastatin A4	Selleck Chemicals	Cat# S7783
Hydroxyurea	Selleck Chemicals	Cat# S1896
Camptothecin	Selleck Chemicals	Cat# S1288
AZD5991	Selleck Chemicals	Cat# S8643
AMG176	MedChemExpress	Cat# HY-101565
A1210477	Selleck Chemicals	Cat# S7790
MIK665	Selleck Chemicals	Cat# S8836
RO-3306	Sigma-Aldrich	Cat# SML0569
Anti-FLAG M2 Magnetic Beads	Sigma-Aldrich	Cat# M8823
Pierce™ Protein A Magnetic Beads	Sigma-Aldrich	Cat# 88845
VECTASHIELD Antifade Mounting Medium with DAPI	Vector Laboratories	Cat# H-1200-10
Critical commercial assays
MycoAlert Mycoplasma Detection Kit	Lonza Biologics	Cat #LT07-218
Pierce Bradford Protein Assay Kit	Thermo Fisher Scientific	Cat# 23238
CellTiter-Glo© Luminescent Cell Viability Assay	Promega	Cat #G7571
Click-iT™ EdU Cell Proliferation Kit	Thermo Fisher Scientific	Cat# C10337
Cell Cycle Control Phospho Antibody Array	Full Moon Biosystems	Cat# PCC238
Duolink® Proximity Ligation Assay	Sigma-Aldrich	Cat# DUO92105
BrdU Cell Proliferation Assay Kit	Cell Signaling Technology	Cat# 6813
Deposited data
Proteomic Data	This paper	ProteomeXchange via the PRIDE database PXD036327
RNA-seq Data	Jain et al.26	NCBI GEO GSE102227
Experimental models: Cell lines
Bax−/−Bak−/− (DKO) p185+Arf−/− B-ALL	Koss et al.38	N/A
Bax−/−Bak−/−Mcl-1−/− (TKO) p185+Arf−/− B-ALL	Koss et al.38; Prew et al.70	N/A
Bax−/−Bak−/−Mcl-1−/−hMCL-1 (TKO+MCL-1) p185+Arf−/− B-ALL	Prew et al.70	N/A
Baxfl/flBak−/− MEF	This paper	N/A
Mcl-1fl/flBaxfl/flBak−/− MEF	This paper	N/A
WT MEF	Perciavalle et al.20	N/A
Mcl-1−/− MEF	Opferman et al.14	N/A
Bax−/−Bak−/− MEF	ATCC	Cat# CRL-2913
Mcl-1fl/flRosa-ERCreT2	Perciavalle et al.20	N/A
Mcl-1fl/flRosa-ERCreT2 + MCL-1	Perciavalle et al.20	N/A
Mcl-1fl/flRosa-ERCreT2 + MCL-1Matrix	Perciavalle et al.20	N/A
Mcl-1fl/flRosa-ERCreT2+ MCL-1OMM	Perciavalle et al.20	N/A
Bax−/−Bak−/− (DKO) MV4;11	This paper; Garciaz et al.71	N/A
HCT116	ATCC	Cat #CCL-247
Bax−/−Bak−/− (DKO) HCT116	Wang et al.72	N/A
H929	ATCC	Cat #CRL-9068
K562	ATCC	Cat #CCL-243
293T	ATCC	Cat #CRL-3216
HeLa	ATCC	Cat #CCL-2
KPNYN	JCRB Cell Bank	Cat# IFO50431
HUCCT1	JCRB Cell Bank	Cat# JCRB0425
Experimental models: Organism/strains
Mouse: C57BL/6J	The Jackson Laboratory	Strain #:000664 RRID:IMSR_JAX:000664
Software and algorithms
Prism v9	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
LAS X	Leica	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
FIJI	ImageJ	https://imagej.net/software/fiji/
FlowJo	BD and Company	https://www.fiowjo.com/solutions/fiowjo
Enrichr	Chen et al.69	https://maayanlab.doud/Enrichr/
Other
DMEM	Thermo Fisher Scientific	Cat# 12491015
RPMI 1640	Thermo Fisher Scientific	Cat #21870-076
Fetal Bovine Serum	GeminiBio	Cat #100–106, Lot #A33H00L
Penicillin and Streptomycin	Gibco	Cat# 15070063
Glutamine	Gibco	Cat# 25030081