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. 2024 Jan 12;22:48. doi: 10.1186/s12967-024-04860-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — TLSs in MIBC. A HE staining shows TLSs (red squares) in MIBC, and IHC staining shows the following markers of TLSs (red squares): CD45, CD19, CD3, MKI67, CD4, CD8, PD-1, CD21, LAMP3, MECA79A and LMO2. Scale bar: 2.5 mm in panoramic images and 100 μm in magnified images. B H&E staining and IHC for the indicated markers for representative LNs (two columns below) and TLSs (two columns above). Scale bar: 2.5 mm in panoramic images and 100 μm in magnified images. C mIF for the indicated markers for representative TLSs. The following markers are shown in individual and merged channels: CD3 (green), T-cell marker; CD19 (red), B-cell marker; DAPI (blue), nuclear marker. Scale bar: 25 μm