Fig. 6.

Transcriptome analysis revealed that T cells recruit B cells via CXCL13-CXCR5 in MIBC. A tSNE plot showing T-cell subtypes. B Average cell numbers of T-cell subtypes in original samples (left). Box plot showing the proportions of CD8_Tex (middle) and CD4_Tex (right) cells in the original samples. C Heatmap indicating the scaled expression of T-cell marker genes. D Bubble plot indicating cell roles: The color of the dots represents different cell groups, the size of the dots is proportional to the number of ligands and receptors inferred for each cell group, and the x and y axes indicate the strength of the cell group as a signal sender and receiver, respectively. E Hierarchy diagram: “Source” represents the cell class that sends the signal, “target” represents the cell class that receives the signal, and the circle color represents the cell class. F The signaling pathway relationship to the bubble diagram shows significant interactions between the ligand‒receptor (L-R) pairs of subpopulations. G The violin diagram shows the expression levels of genes involved in signaling pathways. H Migration of CXCR5 + B cells treated with (middle) or without (left) CXCL13 and the CXCR5 antagonist (right)