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Buffer compositions and volumes for size exclusion chromatography.
| Buffer | CYP11 Proteins | CYP17A1 | CYP21A2 |
|---|---|---|---|
|
| |||
| Size | 50 mM KPi (pH 7.4) | 50 mM KPi (pH 7.4) | 50 mM KPi (pH 6.8) |
| Exclusion | 20% (v/v) glycerol | 20% (v/v) glycerol | 20% (v/v) glycerol |
| Buffer | 500 mM NaCl | 500 mM NaCl | 350 mM NaCl |
| 100 mM glycine | 1 mM EDTA | ||
