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(Top) Restriction map of pJA2 insert; (bottom) Southern hybridization of chromosomal DNA of L. pneumophila digested and probed with the degenerate oligonucleotide corresponding to the N-terminus sequence of the 20-kDa MI protein. Lanes: 1, 1.8-kb BamHI; 2, 11-kb ClaI; 3, 2.2-kb EcoRI; 4, 3.2-kb EcoRV; 5, 8-kb HindIII. The second signal in lane 4 is an artifact. The sizes were based on a 1-kb ladder (BRL).
