Table 2.
Microbial counts (logCFU/mL) in raw and SC-CO2-treated cane juice.
| Trial | Treatment | Coliforms (45 °C) | Mesophiles | Lactic bacteria | Molds and yeasts |
|---|---|---|---|---|---|
| raw | 1.5 ± 0.2 | 3.6 ± 0.1 | 1.0 ± 0.0 | 3.5 ± 0.3 | |
| 1 | 213 bar/50 °C/45 min | <1est | 1.8 ± 0.1 | <1est | 1.1 ± 0.1 |
| log red | >0.5 est | 1.8 | - | 2.4 | |
| raw | <1est | 3.1 ± 0.4 | 2.6 ± 0.3 | 3.5 ± 0.3 | |
| 2 | 130 bar/40 °C/30 min | <1est | 2.7 ± 0.1 | 1.2 ± 0.2 | 1.4 ± 0.2 |
| log red | - | 0.4 | 1.4 | 2.1 | |
| raw | <1est | 4.6 ± 0.3 | 1.3 ± 0.1 | 2.4 ± 0.2 | |
| 3 | 295 bar/40 °C/30 min | <1est | 2.6 ± 0.3 | < 1est | 0.3 ± 01 |
| log red | - | 2.0 | > 0.3 est | 2.1 | |
| raw | 2.5 ± 0.1 | 4.6 ± 0.2 | 3.0 ± 0.2 | 4.2 ± 0.4 | |
| 4 | 130 bar/60 °C/30 min | 0.7 ± 0.2 | 1.6 ± 0.1 | 1.0 ± 0.2 | 1.0 ± 0.1 |
| log red | 1.8 | 3.0 | 2.0 | 3.2 | |
| raw | 1.3 ± 0.3 | 3.7 ± 0.1 | 3.1 ± 0.2 | 3.8 ± 0.5 | |
| 5 | 295 bar/60 °C/30 min | <1est | 1.5 ± 0.1 | 1.5 ± 0.2 | 1.6 ± 0.3 |
| log red | >0.3 | 2.2 | 1.6 | 2.2 | |
| raw | <1est | 4.9 ± 0.3 | 3.0 ± 0.3 | 4.1 ± 0.3 | |
| 6 | 213 bar/50 °C/45 min | <1est | 1.7 ± 0.2 | 1.6 ± 0.3 | 0.9 ± 0.2 |
| log red | - | 3.2 | 1.4 | 3.2 | |
| raw | 2.3 ± 0.1 | 2.7 ± 0.2 | 2.2 ± 0.2 | 4.9 ± 0.3 | |
| 7 | 130 bar/40 °C/60 min | <1est | <1est | 2.1 ± 0.2 | 1.7 ± 0.2 |
| log red | >1.3est | >1.7est | 0.1 | 3.2 | |
| raw | <1est | 6.0 ± 0.3 | 3.5 ± 0.2 | 4.1 ± 0.3 | |
| 8 | 295 bar/40 °C/60 min | <1est | 2.1 ± 0.2 | 1.4 ± 0.2 | 1.8 ± 0.2 |
| log red | - | 3.9 | 2.1 | 2.3 | |
| raw | 3.5 ± 0.3 | 4.7 ± 0.3 | < 1est | 5.4 ± 0.2 | |
| 9 | 130 bar/60 °C/60 min | 2.2 ± 0.1 | 1.7 ± 0.1 | < 1est | 2.6 ± 0.1 |
| log red | 1.3 | 3.0 | - | 2.8 | |
| raw | 5.2 ± 0.1 | 3.0 ± 0.1 | 4.0 ± 0.1 | 4.9 ± 0.6 | |
| 10 | 295 bar/60 °C/60 min | 2.9 ± 0.3 | 2.0 ± 0.3 | 2.2 ± 0.1 | 1.5 ± 0.1 |
| log red | 2.3 | 1.0 | 1.8 | 3.4 | |
| raw | 3.9 ± 0.1 | 2.0 ± 0.2 | 2.1 ± 0.2 | 4.9 ± 0.4 | |
| 11 | 213 bar/50 °C/45 min | 1.4 ± 0.3 | 1.9 ± 0.1 | 0.7 ± 0.1 | 2.7 ± 0.2 |
| log red | 2.5 | 0.1 | 1.4 | 2.2 | |
| raw | 3.3 ± 0.1 | 5.5 ± 0.6 | 0.7 ± 0.2 | 4.8 ± 0.4 | |
| 12 | 74 bar/ 50 °C/ 45 min | 2.3 ± 0.4 | 3.2 ± 0.2 | 0.4 ± 0.2 | 1.9 ± 0.1 |
| log red | 1.0 | 2.3 | 0.3 | 2.9 | |
| raw | 3.4 ± 0.2 | 5.6 ± 0.1 | 3.9 ± 0.2 | 4.2 ± 0.4 | |
| 13 | 351 bar/50 °C/45 min | 2.5 ± 0.2 | 3.6 ± 0.3 | 2.8 ± 0.2 | 1.8 ± 0.3 |
| log red | 0.9 | 2.0 | 1.1 | 2.4 | |
| raw | 3.3 ± 0.1 | 5.6 ± 0.3 | 1.2 ± 0.1 | 5.1 ± 0.5 | |
| 14 | 213 bar/33 °C/45 min | 2.8 ± 0.2 | 2.8 ± 0.1 | < 1est | 2.6 ± 0.3 |
| log red | 0.5 | 2.8 | > 0.2est | 2.5 | |
| raw | 1.8 ± 0.2 | 5.9 ± 0.5 | 2.5 ± 0.4 | 4.7 ± 0.1 | |
| 15 | 213 bar/67 °C/45 min | <1est | 4.1 | < 1est | 0.6 ± |
| log red | >0.8est | 1.8 | 0.0 | 4.1 | |
| raw | 2.8 ± 0.3 | 5.3 ± 0.5 | 3.7 ± 0.1 | 5.3 ± 0.2 | |
| 16 | 213 bar/50 °C/20 min | 0.8 ± 0.1 | 3.2 ± 0.2 | 3.5 ± 0.3 | 1.8 ± 0.2 |
| log red | 2.0 | 2.1 | 0.2 | 3.5 | |
| raw | 2.9 ± 0.2 | 4.8 ± 0.1 | 3.6 ± 0.3 | 4.3 ± 0.2 | |
| 17 | 213 bar/50 °C/70 min | 0.8 ± 0.1 | 2.8 ± 0.2 | 2.8 ± 0.1 | 1.6 ± 0.3 |
| log red | 2.1 | 2.0 | 0.8 | 2.7 |
Mean values of 3 replicates ± standard deviation.
est – estimate count (under detection limit).