TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| E. coli χ6212 | Φ80d lacZ ΔM15 deoR Δ(lacZYA-argF)U169 supE44 λ− gyrA recA1 relA1 endA1 ΔasdA4 Δ[zhf-2::Tn10] hsdR17 (r− m+) | R. Curtiss III |
| S. typhimurium | ||
| χ4072 SR-11 | pStSR100−gyrA1816 Δcya-1 Δcrp-1 ΔasdA1 Δ(zhf-4::Tn10) | 24 |
| χ4217 SR-11 | pStSR101+gyrA1816 Δcya-1 Δcrp-1 ΔasdA1 Δ(zhf-4::Tn10) | 33 |
| χ3985 UK-1 | pStV1+ Δcya-12 Δ(zid-62::Tn10) Δcrp-11 Δ(zhb::Tn10) | 7 |
| χ3987 UK-1 | pStV1+ Δcya-12 Δ(zid-62::Tn10) Δcrp-11 Δ(zhc-1431::Tn10) ΔasdA1 Δ(zhf-4::Tn10) | 14 |
| χ4217-10E | χ4217 with insertion of mini-Tn5 asd elt-K63 | 2 |
| χ3987-18A | χ3987 with insertion of mini-Tn5 asd elt-K63 | 2 |
| Plasmids | ||
| pBluescript KS-LT | E. coli vector with LT gene inserted into the SmaI-HindIII sites | 28 |
| pBluescript KS-LTK63 | E. coli vector with mutant LTK63 gene inserted into the SmaI-HindIII sites | 28 |
| pYA3074 | Low-copy-number Asd+ vector with pSC101 replicon | R. Curtiss III |
| pYA3149 | Medium-high-copy-number Asd+ vector with pBR322 replicon | R. Curtiss III |
| pYA3137 | High-copy-number Asd+ vector with pUC replicon | R. Curtiss III |