Table 3.

Mean and standard error (SEM) of serum biochemistry of steers supplemented with amylase and blend enzymes.

Items	Treatments1				SEM	P – values
	T1 (n = 6) Control	T2 (n = 6) Blend	T3 (n = 6) Amylase	T4 (n = 6) Blend+Amylase		Treat	Treat × Day
Albumin (g/dL)						0.88	0.45
d 1	6.36	5.86	6.07	5.58	0.29
d 15	2.50	2.63	2.64	2.78	0.29
d 60	2.76	2.83	2.52	3.40	0.29
d 120	3.05	3.01	2.89	2.90	0.29
Average2	2.89	2.79	2.72	2.91	0.14
Globulin (g/dL)						0.48	0.20
d 1	3.30	2.91	3.51	3.21	0.79
d 15	6.02	5.81	5.99	5.71	0.79
d 60	9.12	11.8	12.3	9.99	0.79
d 120	4.15	4.99	3.9	5.14	0.79
Average2	6.45	7.43	7.48	6.94	0.53
Total protein (g/dL)						0.73	0.36
d 1	9.68	8.74	9.61	8.78	0.83
d 15	8.53	8.41	8.65	8.48	0.83
d 60	11.9	14.6	14.8	13.4	0.83
d 120	7.21	7.98	6.8	8.03	0.83
Average2	9.38	10.2	10.3	9.81	0.57
Glucose (mg/dL)						0.06	0.86
d 1	97.7	98.6	97.0	93.9	6.37
d 15	84.8	77.1	82.4	87.7	6.37
d 60	105	86.6	89.4	106	6.37
d 120	96.4	72.9	85.4	84.9	6.37
Average2	95.5a	78.9c	85.7bc	92.6ab	3.24
Urea (mg/dL)						0.98	0.18
d 1	23.6	20.8	22.6	21.6	1.87
d 15	26.2	23.3	22.9	23.3	1.87
d 60	26.6	25.3	25.6	28.1	1.87
d 120	27.1	31.7	32.1	28.4	1.87
Average2	27.1	26.3	27.1	26.4	1.51
Cholesterol (mg/dL)						0.75	0.51
d 1	108	97.6	86.8	97.2	11.7
d 15	103	87.1	90.4	117	11.7
d 60	98.6	106	97.1	103	11.7
d 120	116	113	138	125	11.7
Average2	109	102	105	115	9.40

¹Treatments were: T Control- Control treatment, T2 blend– Treatment with 0.5 g of blend per kg of DM in the diet, T3 Amylase- Treatment with 0.5 g of amylase per kg of DM in the diet, T4 blend + Amylase- Treatment with 0.5 g enzyme blend + 0 0.5 g amylase per kg of DM in the diet.

²The d 1 results were removed from the data set to generate the average per treatment in the statistical analysis.

^a,b,c Note: P ≤ 0.05 (different) and P ≥ 0.05 to ≤ 0.1 (trend) were illustrated by different letters (a,b,c) on the same line.