. 2023 Dec 17;41:e00824. doi: 10.1016/j.btre.2023.e00824

Table 5.

Mean and standard error (SEM) of total short-chain fatty acids (SCFA) in ruminal fluid and profile of volatile fatty acids in the rumen of cattle that consumed exogenous enzymes.

Variables	T1 (n = 6) Control	T2 (n = 6) Blend	T3 (n = 6) Amylase	T4 (n = 6) Blend+Amylase	SEM	P- trat	P- trat x day
Acetic acid (mmol/L						0.05	0.05
d60	64.7^ab	51.5^b	58.2^ab	69.0^a	1.98
d120	68.4^b	77.2^a	75.1^ab	77.9^a	1.57
Average	66.5^b	64.3^b	66.6^b	73.4^a	1.45
Propionic acid (mmol/L)						0.01	0.01
d60	15.6^bc	13.7^c	17.0^ab	19.7^a	0.74
d120	18.0^b	26.9^a	29.4^a	28.8^a	0.81
Average	16.8^b	20.3^ab	23.2^a	24.2^a	0.75
Butiric acid (mmol/L)						0.06	0.04
d60	10.9	10.81	10.6	12.6	0.65
d120	13.3^b	15.5^ab	15.6^ab	17.1^a	0.66
Average	12.1^b	13.1^ab	13.1^ab	14.9^a	0.62
Isovaleric acid (mmol/L)						0.59	0.23
d60	1.82	1.60	1.49	1.70	0.11
d120	1.72	2.23	1.74	2.05	0.09
Average	1.77	1.91	1.61	1.87	0.1
Valeric acid (mmol/L)						0.44	0.62
d60	1.77	1.51	1.59	1.85	0.21
d120	2.01	2.50	2.35	2.61	0.36
Average	1.89	2.00	1.97	2.23	0.25
SCFA (mmol/L)						0.01	0.01
d60	94.7^b	79.1^c	88.8^b	104.8^a	2.04
d120	103.4^b	124.3^a	124.2^a	128.4^a	1.96
Average	99.0^b	101.7^b	106.5^b	116.6^a	2.01

¹Treatments were: T1 Control- Control treatment, T2 blend– Treatment with 0.5 g of blend per kg of DM in the diet, T3 Amylase- Treatment with 0.5 g of amylase per kg of DM in the diet, T4 blend + Amylase- Treatment with 0.5 g enzyme blend+ 0 0.5 g amylase per kg of DM in the diet.

²The d 1 results were removed from the data set to generate the average per treatment in the statistical analysis.

^a,b,c Note: P ≤ 0.05 (different) and P ≥ 0.05 to ≤ 0.1 (trend) were illustrated by different letters (a,b,c) on the same line.