FIG. 8.

Increasing the bacterial inoculum does not increase surface E-selectin on cytochalasin D-treated cells. HUVEC were incubated with 250 μg of cytochalasin D/ml for 60 min (black bars) or left untreated (hatched bars) prior to infection with twofold increments of L. monocytogenes EGD. The mean (±SD) inoculum (1×) was 1.72 × 10³ ± 0.23 × 10³ CFU of bacteria/well. Cells were cultured for 60 min, gentamicin was added, and the cells were incubated for another 4 h. E-selectin expression was measured by whole-cell ELISA. Results presented are the mean (±SEM) absorbance at 490 nm from three experiments.