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. 2023 Aug 4;31(1-2):31–44. doi: 10.1038/s41434-023-00414-0

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© The Author(s) 2023

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Fig. 2 — A Detection of th mRNA and the components of the SAM system as determined by RT-PCR in astrocytes; lane 1- H2O (negative control), lane 2- astrocytes, lane 3- astrocyte-TH cells. B Detection of Th protein as determined by Western blot analysis; lane 1- astrocytes, lane 2- astrocyte TH, and lane 3- rat striatum. In A and B, actin is a positive control. C Determination of DA in the extracellular medium, as determined by HPLC in astrocytes and astrocyte-TH cells; independent t-test p = 0.01, n = 3. D Immunofluorescence in astrocytes for Th, GFAP, and DAPI (Nuclei). E Immunofluorescence for Th, GFAP, and DAPI in astrocyte-TH cells. Images in D and E were obtained with confocal microscopy. Bars represent 30 µm.