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. 2024 Jan 15;12:2. doi: 10.1038/s41413-023-00299-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Preparation and characterization of the MSN+miR-146a complex. a–c ECM and TEM images and EDS of MSNs. d TEM images of MSN-PEI. e CCK-8 assay of hDPSCs after 8 and 24 h of coculture with PEI-modified MSNs at various concentrations. f, g Gel retardation and zeta potential tests of the MSN+miR-146a complex at different weight ratios. h–j Cellular uptake assay of MSN+miR-146a-FAM in hDPSCs after 24 h of coculture. Cells were stained to label the cytoskeleton, cell membrane or lysosome. Yellow arrows indicate colocalization of lysosomes and MSN+miR-146a-FAM. ns no significance. One-way ANOVA with Dunnett’s multiple comparisons to the blank group was used