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. 2024 Jan 15;9:18. doi: 10.1038/s41392-023-01709-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Loss of Bim reduced Ube2m deletion-induced apoptosis of DN T cells. a The number of splenocytes was calculated. n = 5/group. *P < 0.05, **P < 0.01, ***P < 0.001. b The number of T cells in spleens was measured. n = 5/group. **P < 0.01, ***P < 0.001. c CD3⁺ T cell subsets in spleens were analyzed using flow cytometry. n = 3/group. d, e The percentage and number of T cell subsets were quantified and calculated based on the flow cytometry data. n = 3/group. *P < 0.05, **P < 0.01. f–h The apoptosis (Annexin V⁺/7-AAD^-) of total T cells, DN T cells, CD4⁺ and CD8⁺ T cells was evaluated and quantified. n = 3/group. *P < 0.05, **P < 0.01, ***P < 0.001