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. 2023 Dec 20;10(1):e23870. doi: 10.1016/j.heliyon.2023.e23870

Fig. 2.

Fig. 2

Melatonin triggers ROS-dependent apoptosis in breast tumor cells. A. ROS generation in breast tumor cells isolated from experimental animals. (i) Histogram showing merged intensities of DCF-DA, (ii) Graphical representation of DCF-DA intensities proportional to the intracellular ROS levels in breast tumor cells isolated from experimental animals analyzed on flow cytometer. B. Melatonin-induced apoptosis of breast tumor cells as analyzed by Annexin V-FITC assay using a flow cytometer. (i) Percentage of apoptotic cells analyzed in control and treated groups and (ii) corresponding graphical representation of percentage of early and late apoptotic cells in control and treated groups. C. Representative images of apoptotic cell death and nuclear morphology in DAPI-stained EAC cells isolated from experimental animals (fluorescence microscopy). White arrow indicates fragmented DNA; magnification: 40X. D. Effect of melatonin on cell death and ROS generation in EAC cells. Spectrofluorimetric detection of ROS and MTT assay for assessment of cell viability of cultured EAC cells under different conditions.