Skip to main content
. 2023 Nov 20;300(1):105486. doi: 10.1016/j.jbc.2023.105486

Figure 1.

Figure 1

The effect of tACE on sperm metabolites.A, heatmap showing metabolites that are significantly changed (p-value < 0.01) among two groups: CKO versus WT and WT+Ram versus WT. Mean z-scores were created for each protein using GraphPad Prism version 7.04. The mean data for all metabolites are listed in Table S1. WT+Ram groups of mice were treated with 40 mg/l ramipril (Ram) for 1 week before sperm isolation. B, volcano plots showing the metabolites that are significantly changed in CKO versus WT, WT+Ram versus WT and CKO versus WT+Ram. The blue dots represent downregulated metabolites. The differentially expressed metabolites were sorted with the criteria of p-value < 0.01 and Fold Change > 5. C, differential cellular levels of ATP, ADP, AMP, and adenosine in WT, CKO, and WT+Ram sperm. A–C, metabolite array was performed using mass spectrometry (n = 5/group). D, biochemical analysis of ATP production in WT and CKO sperm. Indicated groups of mice were treated with either ramipril (40 mg/l) or losartan (600 mg/l) for a week before sperm isolation. A–D, a one-way ANOVA with Bonferroni’s correction for multiple comparisons was used to analyze group comparisons, and data are presented as means ± SEM. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001. CKO, C-domain KO; tACE, testis angiotensin-converting enzyme.