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. 2023 Nov 20;300(1):105486. doi: 10.1016/j.jbc.2023.105486

Figure 2.

Figure 2

The effect of tACE on mitochondrial protein in sperm.A, heat map showing the level of mitochondrial proteins in WT and CKO sperm measured by the MitoPlex assay. Mean z-scores were created for each protein using GraphPad Prism version 7.04. Data are from the analysis of sperm (5 mice/group). The mean data for all proteins are listed in Table S2. B, volcano plot showing differential level of proteins between CKO and WT sperm. The blue dots represent downregulated mitochondrial proteins. The significantly different proteins are sorted according to the criteria of p-value < 0.05. C, measurement of selected mitochondrial proteins by Western blot analysis. Data are presented as means ± SEM (n = 10/group). ∗p < 0.05; ∗∗∗p <0.001 determined by two-tail student t test. D, KEGG pathway and GO analyses of mitochondrial proteins using the MitoPlex array. A significant difference between WT and CKO sperm is determined by the p-value less than 0.05. CKO, C-domain KO; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; tACE, testis angiotensin-converting enzyme.