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. 2023 Nov 20;300(1):105486. doi: 10.1016/j.jbc.2023.105486

Figure 5.

Figure 5

tACE induces mitochondrial respiration.A, oxygen consumption rates (OCR) of WT or CKO sperm measured with an Agilent MitoXpress oxygen consumption assay. The graph shows the trace of OCR in sperm under basal conditions and in response to mitochondrial effectors oligomycin, FCCP, and antimycin A/rotenone. B, the rates of ATP production from glycolysis (ATPGlyco.) and oxidative phosphorylation (ATPOxPhos.) in WT and CKO sperm. C, OCR of maximal respiration (n = 10/group). D, extracellular acidification rate (ECAR) of WT or CKO sperm. The data show the trace of ECAR in sperm under basal conditions and in response to glucose, oligomycin, and 2-deoxy-d-glucose (2DG). E and F, ECAR of glycolysis (E) and glycolytic capacity (F) (n = 12 per group). A–F, groups of samples were treated with 10 μM GW9662 for 12 h before analysis as indicated. Data are presented as means ± SEM. An one-way ANOVA with Bonferroni’s correction for multiple comparisons was used to analyze group comparisons. NS, no significance. ∗∗p < 0.01 and ∗∗∗p < 0.001. CKO, C-domain KO; ECAR, extracellular acidification rate; FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; tACE, testis angiotensin-converting enzyme.