Figure 7.

The metabolic and physiologic effect tACE in human sperm. Human sperm were treated for 12 h with either 10 μM GW9662 or 10 μM ramipril or 100 μM losartan and then (A) production of ATP, (B) motility, and (C) acrosine activity were determined as described in the Experimental procedures. Untreated samples were used as a control. Sperm representative motility video is shown in Video S2. Data are presented as means ± SEM (n = 13/group). An one-way ANOVA with Bonferroni’s correction for multiple comparisons was used to analyze group comparisons. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. tACE, testis angiotensin-converting enzyme.