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. 2023 Dec 10;300(1):105554. doi: 10.1016/j.jbc.2023.105554

Figure 3.

Figure 3

Outer membrane import differentially affects trimeric and nontrimeric enterobactin (Ent)-associated exometabolites in culture. Ent-associated exometabolite concentrations in media conditioned by UTI89 (wildtype), an import-deficient UTI89 mutant (ΔtonB), or coculture of Ent-null and import-deficient UTI89 mutants (ΔtonB + ΔentB). Y-axis is concentration expressed as ratio of LC–MS/MS peak area to that of internal standard. (A) Ent. (B) lin-Ent. (C) MGE. (D) lin-MGE. (E) DGE. (F) lin-DGE. (G) (DHBS)2. (H) G1-(DHBS)2. (I) G2-(DHBS)2. (J) DHBS. Data are presented as mean ± SD with at least three biologically independent samples. Statistics were performed using one-way ANOVA with Dunnett’s multiple-comparison test with p ≤ 0.05 considered as statistically significant. ns, not significant; ∗p ≤ 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.