Table 1.
Targeted LC–MS/MS protocols for detecting and quantifying 10 Ent siderophores and short-length products
| Identity | Selected ions (m/z) |
Retention time (min) | References | |
|---|---|---|---|---|
| Q1a | Q3b | |||
| Ent | 668 | 222 | 8.47 | (21, 46, 51, 86, 90, 91) |
| lin-Ent | 686 | 222 | 7.21 | (21, 46, 47, 51) |
| MGE | 832 | 222 | 6.94 | (46, 47, 51) |
| lin-MGE | 848 | 222 | 5.89 | (46, 47, 51) |
| DGE | 993 | 222 | 5.95 | (46, 47, 51, 92) |
| lin-DGE | 1010 | 222 | 4.98 | (21, 46, 47, 51, 92) |
| (DHBS)2 | 464 | 222 | 5.92 | (46, 51, 86, 90, 91) |
| G1-(DHBS)2 | 625 | 222 | 4.69 | (46, 47, 51, 92) |
| G2-(DHBS)2 | 787 | 402 | 3.64 | (46, 47, 51) |
| DHBS | 240 | 153 | 2.34 | (46, 51, 86, 90, 91) |
a
Q1 represents the m/z of the precursor ion selected by the first quadrupole during MS/MS.
b
Q3 represents the m/z of the fragment ion selected by second quadrupole during MS/MS.