Figure 2.

Levels of activated FXI were elevated in a model of hyperlipidemia. PPP was isolated from NHPs on a standard chow diet (n = 6, lean) or NHPs on a high-fat diet (n = 8, obese). Clotting times were measured by incubating PPP with an aPTT reagent followed by CaCl₂ to initiate clot formation (A) or by stimulating PPP with Dade Innovin Reagent to measure PT. Activated coagulation protease species, FXIa–AT and T-AT, were measured using a custom ELISA (C, D). Statistical analyses were conducted using a Mann–Whitney test or an unpaired t-test. Statistical significance is indicated by an asterisk for P ≤ .05. Data are shown as mean ± SEM. aPTT, activated partial thrombospondin time; AT, antithrombin; ELISA, enzyme-linked immunosorbent assay; F, factor; PPP, platelet-poor plasma; PT, prothrombin time; NHP, nonhuman primate.