Figure 4.

FXI inhibition prolonged aPTT clotting times in a model of hyperlipidemia. PPP was isolated from NHPs on a high-fat diet (n = 5) treated with a FXI function–blocking antibody. Clotting times were measured by incubating PPP with an aPTT reagent followed by CaCl₂ to initiate clot formation (A) or by stimulating PPP with Dade Innovin Reagent to measure PT (B). Activated coagulation protease species, FXIa–AT and T–AT, were measured using a custom ELISA (C, D). Statistical analyses were conducted using a Friedman test with a Dunn’s post-hoc test or repeated measures one-way ANOVA with a Dunnett’s post-hoc test. Statistical significance is indicated by an asterisk for P < .05. Data are shown as mean ± SEM. aPTT, activated partial thrombospondin time; AT, antithrombin; ELISA, enzyme-linked immunosorbent assay; F, factor; NHP, nonhuman primate; PPP, platelet-poor plasma; PT, prothrombin time.