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. 2024 Jan 1;15(4):966–980. doi: 10.7150/jca.89135

Figure 3.

Figure 3

circFAM126A promotes PCa in vitro. sh-circFAM126A was transfected into PC-3 and DU145 cells to study its effects. A: The knockdown efficiency of sh-circFAM126A#1 and sh-circFAM126A#2 on circFAM126A was evaluated using RT-qPCR. B: The cell proliferation rate was measured using the CCK-8 assay. C: Colony formation assays were performed to assess cell proliferation capability. D: EdU assay was utilized to determine the Edu-positive rate in cells. E: Flow cytometry was employed to analyze cell apoptosis. F and G: Transwell assays were conducted to investigate the cell migration and invasion abilities. H and I: TG and cholesterol levels in cells were measured using commercial assay kits. J and K: The mRNA levels of LUR1 and SREBPs in cells were detected via RT-qPCR. Data are presented as mean ± SD (n=3), with * P < 0.05 indicating statistical significance.