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. 2024 Jan 1;15(4):966–980. doi: 10.7150/jca.89135

Figure 4.

Figure 4

m6A modification of circFAM126A improves transcriptome stability. A: The bioinformatics tool m6A Avar revealed the presence of m6A modification sites on CircFAM126A. B: MazF-PCR was employed to assess the abundance of m6A-modified CircFAM126A in cells. C: The expression levels of methylation-related proteins were quantified using RT-qPCR. D: RT-qPCR was utilized to measure the mRNA levels of IGF2BP1 in 46 paired PCa samples. E: Western Blot analysis was conducted to evaluate the protein expression of IGF2BP1 in 46 paired PCa samples. F: RNA pull-down assays were performed to investigate the targeted binding relationship between IGF2BP1 and CircFAM126A. G: RIP assays were employed to confirm the targeted interaction between IGF2BP1 and CircFAM126A. H: The effects of IGF2BP1 knockdown on IGF2BP1 and CircFAM126A levels in cells were assessed using RT-qPCR. I: Pearson correlation analysis demonstrated a positive correlation between CircFAM126A and IGF2BP1 mRNA levels in the tumor tissues of 46 PCa patients. Data are presented as mean ± SD (n = 3), with * P < 0.05.