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. 2024 Jan 1;15(4):966–980. doi: 10.7150/jca.89135

Figure 7.

Figure 7

circFAM126A sponges miR-505-3p to upregulate CANX. A: Bioinformatics websites PITA, microT, TargetScan, PicTar, and the miRanda database were utilized to predict potential downstream mRNA targets of miR-505-3p. B and C: The impact of knocking down or overexpressing miR-505-3p on the mRNA and protein expression of CANX was examined using RT-qPCR and Western blot. D: Dual-luciferase reporter assay was conducted to investigate the target relationship between miR-505-3p and CANX. E: The bioinformatics website starbase was employed to predict potential binding sites between CANX and miR-505-3p. F: Biotinylated pull-down assay was used to analyze the targeting relationship between miR-505-3p and CANX. G and H: RT-qPCR and Western blot assays were carried out to measure the mRNA and protein expression of CANX in PCa tissues and adjacent normal tissues. I and J: Pearson correlation analysis was performed to assess the relationship among CircFAM126A, miR-505-3p, and CANX in cancer tissues. K and L: The effect of knocking down CircFAM126A on the mRNA and protein levels of CANX was detected using RT-qPCR and Western blot. Data are presented as mean ± SD (n = 3), with * P < 0.05 indicating statistical significance.