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(a) Special double-bladed trephine (Jacob CAIRS trephineTM, Madhu Instruments, India); (b) CAIRS Trephine used to punch allogenic tissue (here, donor cornea denuded of epithelium and endothelium); (c) Bowman's membrane (BM) side of CAIRS inked using surgical marker pen to facilitate later side identification; (d) Circular CAIRS removed from trephine and cut to obtain strip of tissue
