Figure 7.

ATP potentiates Meropenem combat against CRAB persisters, biofilm, and systemic infections. (A) Viability of Ab4 after incubation with an increasing concentration of ofloxacin (0–960 μg/mL). (B) Viability of Ab4 persists in the presence or absence of Meropenem plus ATP for 6 h in vitro. (C) Viability of the Ab4 biofilm in the presence or absence of Meropenem and ATP for 6 h in vitro. (D) Percent survival of mice intraperitoneally challenged with 7 × 10⁷ cfu Ab4 and then treated with saline solution, ATP (3 mg/kg), Meropenem (100 mg/kg), or both 1 h after infection (n = 10 per group). (E) Bacterial load in mice blood, spleen (F), liver (G), and kidney (H) after treatment with saline solution, ATP (3 mg/kg), Meropenem (100 mg/kg), or both for 6 h (n = 5 per group). Results are displayed as means ± SD. Significant are identified *P < 0.05 and **P < 0.01.