Figure 4.

Determination of trophozoites growth inhibition with various concentrations of six different compounds in A. castellanii culture. Cell density was determined by optical scanning of crystal violet staining (absorbance) at three-time points (24, 48, and 72 h). Unpaired t-test was used to compare each concentration against the control (0), at the 72 h time point (bold: p-value < 0.05). All tested inhibitors, except brinzolamide, were discovered to decrease the viability of the trophozoites. Propamidine is presented as percentage values because the eye drop used is 0.1% concentration. The number of sample replicates were 11 for acetazolamide, 11 for brinzolamide, 15 for dorzolamide, 6 for ethoxzolamide, 7 for Fc14-584B and 6 for propamidine. Whiskers represent standard deviation (SD).