Figure 6.

Excystation assay indirectly displays the ability of cysts to excystate and act as an active trophozoite with various concentrations of six different compounds in A. castellanii culture. Cell density was determined by optical scanning of crystal violet staining (absorbance) at one-time point (72 h). Unpaired t-test was used to compare each concentration against the control (0) (*: p-value < 0.05). All tested inhibitors were discovered to decrease the ability of the cysts to excystate. Propamidine is presented as percentage values because the eye drop used is 0.1% concentration in the presence of inhibitor. The number of sample replicates was 6 for each inhibitor. Whiskers represent standard deviation (SD).