Figure 8.

Phylogenetic inference shows the relatedness of A. castellanii and endosymbiont β-CAs. A total of 3 A. castellanii and 95 endosymbiont β-CA protein sequences were identified by BLAST search and subsequently analyzed for relatedness by maximum likelihood-based phylogenetic inference, with IQ-TREE.⁴⁴ The resulting consensus tree was visualized with the ETE toolkit⁴⁵ (vers. 3.1.2). Organism phyla are indicated by colors, as defined in the legend, and circle size at nodes represents the percentage of replicates supporting that branch topology.