Figure 9.

Phylogenetic inference shows the relatedness of A. castellanii and endosymbiont γ-CAs. A total of 2 A. castellanii and 46 endosymbiont γ-CA protein sequences were identified by BLAST search and subsequently analyzed for relatedness by maximum likelihood-based phylogenetic inference, with IQ-TREE.⁴⁴ The resulting consensus tree subsequently visualized with the ETE toolkit⁴⁵ (vers. 3.1.2). Organism phyla are indicated by colors, as defined in the legend, and circle size at nodes represent the percentage of replicates supporting that branch topology.