TABLE 1.
Characteristics of recombinant plasmids
| Plasmid | Characteristic(s) | Reference or source |
|---|---|---|
| pUC18 | AprlacZ′ ColE1 ori | 58 |
| pBluescriptKS+ | AprlacZ′ f1 ori ColE1 ori PT7 PT3 | Stratagene |
| pGEM7Zf(−) | AprlacZ′ f1 ori ColE1 ori PT7 PSP6 | Promega |
| pVS520 | pUB1601 derivative, Tcr Tra+ Kms | 39 |
| pMMB67EH | RSF1010 derivative, AprlacIq Ptac | 19 |
| pMMB67HE | pMMB67EH with polylinker in opposite orientation | 19 |
| p19A | pTZ19R carrying pilBCD on a 4.0-kb XbaI fragment | D. Nunn, University of Washington |
| pUC19Ω | pUC19 carrying Ω on a 2.0-kb SmaI fragment | 29 |
| pJIR922 | pBSKS carrying the fimNOP gene region on a 4.6-kb BamHI fragment | 25 |
| pJIR1003 | pBSKS carrying part of fimN on a 2.3-kb EcoRI/BamHI fragment | Recombinant |
| pJIR1042 | pMMB67EH carrying fimP on a 2.8-kb HpaI fragment | Recombinant |
| pJIR1053 | pBSKS carrying fimO, fimP, and ORF197 on a 3.7-kb PstI fragment | Recombinant |
| pJIR1068 | pMMB67EH carrying fimO on a 1.8-kb insert | Recombinant |
| pJIR1118 | pMMB67HE carrying pilC on a 2.2-kb EcoRI/BamHI fragment | Recombinant |
| pJIR1168 | pUC18 carrying fimN on a 3.2-kb insert | Recombinant |
| pJIR1204 | pGEM7Zf carrying fimP on a 1.7-kb insert | 25 |
| pJIR1205 | pMMB67EH carrying ΔfimP and ORF197 on a 1.9-kb insert | Recombinant |
| pJIR1251 | pUC18 carrying fimN on a 1.8-kb insert | Recombinant |
| pJIR1262 | pMMB67EH carrying pilB on a 2.0-kb insert | Recombinant |
| pJIR1263 | pMMB67HE carrying Ω insertion mutant of fimP on a 3.7-kb insert | Recombinant |
| pJIR1332 | pMMB67HE carrying fimN on a 1.8-kb insert | Recombinant |
| pJIR1401 | pUC18 carrying pilBN fusion on a 2.0-kb insert | Recombinant |
| pJIR1405 | pMMB67EH carrying pilB183-fimN fusion on a 2.0-kb insert | Recombinant |
| pJIR1406 | pMMB67EH carrying fimN and fimO on a 3.6-kb insert | Recombinant |