Table 1.
Antibodies used in Western blot experiments
| Protein | Dilution | Supplier (catalog #) |
|---|---|---|
| Mouse primary antibodies | ||
| AT270 (pThr181) | 1/1,000 | Thermo-Fisher (#MN1050) |
| CP13 (pSer202) | 1/1,000 | Peter Davies |
| AT100 (pThr212/pSer214) | 1/1,000 | Thermo-Fisher (#MN1060) |
| MC6 (pSer235) | 1/1,000 | Peter Davies |
| PHF1 (pSer396/pSer404) | 1/1,000 | Peter Davies |
| GSK3β | 1/1,000 | BD Biosciences (#610202) |
| Demethylated PP2AC | 1/1,000 | Santa Cruz (sc-13601) |
| PP2A, C subunit | 1/1,000 | Millipore (#05-545) |
| β-actin | 1/10,000 | Sigma-Aldrich (#A2228) |
| Rabbit primary antibodies | ||
| pSer199 | 1/1,000 | Thermo-Fisher (#44734G) |
| pThr205 | 1/1,000 | Thermo-Fisher (#44738G) |
| pSer262 | 1/1,000 | Thermo-Fisher (#44750G) |
| pSer409 | 1/1,000 | Thermo-Fisher (#44760G) |
| pSer422 | 1/1,000 | Thermo-Fisher (#44774G) |
| Total tau (TauC) | 1/10,000 | Dako Cytomation (#A0024) |
| pGSK3β (Ser9) | 1/1,000 | Cell Signaling (#9336) |
| HSP70 | 1/1,000 | Cell Signaling (#4872S) |
| HSP90 | 1/1,000 | Cell Signaling (#4877S) |
| SAPK/JNK | 1/1,000 | Cell Signaling (#9252S) |
| pThr183/Tyr185 SAPK/JNK | 1/1,000 | Cell Signaling (#9251S) |
| Secondary antibodies | ||
| Goat anti-mouse IgG HRP conjugate (HC+LC) | 1/5,000 | Jackson laboratories (#115-035-003) |
| Goat anti-mouse IgG HRP conjugate (LC specific Ab) | 1/5,000 | Millipore (#AP200P) |
| Goat anti-rabbit IgG HRP conjugate (HC+LC) | 1/5,000 | Jackson laboratories (#111-035-144) |