Figure 2.

The percentage of double-positive T cells expressing VISTA/PD-1/Tim-3/TIGIT in PB from patients with AL amyloidosis and HIs. (A) The analytic logic of flow cytometry detection of VISTA/PD-1/Tim-3/TIGIT co-expression within CD3⁺, CD4⁺, and CD8⁺ T cell subsets in PB. (B) Comparison of the double-positive T cells co-expressing VISTA/PD-1/Tim-3/TIGIT in PB between patients with AL amyloidosis (n = 19) and HIs (n = 19). AL = amyloid light chain, HIs = healthy individuals, PB = peripheral blood, PD-1 = programmed cell death-1, TIGIT = T cell immunoreceptor with Ig and ITIM domains, Tim-3 = T cell immunoglobulin and mucin-domain-containing-3, VISTA = V-domain immunoglobulin suppressor of T cell activation.