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. 2024 Jan 15;6(1):e00181. doi: 10.1097/BS9.0000000000000181

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Copyright © 2024 The Authors. Published by Wolters Kluwer Health Inc., on behalf of the Chinese Medical Association (CMA) and Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College (IHCAMS).

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 3. — The percentage of VISTA/PD-1/Tim-3/TIGIT⁺ Treg in PB and BM from patients with AL amyloidosis and HIs. (A) The analytic logic of flow cytometry detection of single positive (VISTA or PD-1 or Tim-3 or TIGIT) Treg or double-positive (co-expression with each other) Treg in PB or BM. (B) Comparison of the percentage of VISTA/PD-1/Tim-3/TIGIT⁺ Tregs in PB between patients with AL amyloidosis (n = 19) and HIs (n = 19). (C) Comparison of the percentage of VISTA/PD-1/Tim-3/TIGIT⁺ Tregs in BM between patients with AL amyloidosis (n = 19) and HIs (n = 10). AL = amyloid light chain, BM = bone marrow, HIs = healthy individuals, PB = peripheral blood, PD-1 = programmed cell death-1, TIGIT = T cell immunoreceptor with Ig and ITIM domains, Tim-3 = T cell immunoglobulin and mucin-domain-containing-3, VISTA = V-domain immunoglobulin suppressor of T cell activation.