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. Author manuscript; available in PMC: 2024 Jan 15.
Published in final edited form as: Cell Rep. 2023 May 11;42(5):112508. doi: 10.1016/j.celrep.2023.112508

Figure 2. HPV E5 downregulated key antigen-processing molecules, immunoproteasome, and TAP.

Figure 2.

(A–C) Transcripts and proteins in HPV E5-expressing CAL-27 and DC2.4 cells were analyzed.

(A) mRNA expression of immunoproteasome subunits analyzed by qPCR.

(B) Western blot of immunoproteasome subunits. An asterisk indicates a non-specific signal.

(C) mRNA expression of TAP analyzed by qPCR.

(D) Immunoproteasome activities in HPV E5-expressing CAL-27 and DC2.4 cells were analyzed using fluorescence-conjugated substrates. Left: average of fluorescent units (FUs) per minute throughout the time monitored. Right: fluorescence kinetics of three substrates. Data are represented as mean ± SEM (n = 3).