KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-LMP2 (clone G-3) | Santa Cruz Biotechnology | Cat#: sc-373996; RRID: AB_10918476 |
| Mouse monoclonal anti-LMP7 (clone D-2) | Santa Cruz Biotechnology | Cat#: sc-374089; RRID: AB_10947104 |
| Mouse monoclonal anti-α-tubulin | Developmental Studies Hybridoma Bank (DSHB) | Cat#: 12G10; RRID: AB_2315509 |
| Rabbit polyclonal anti-STAT1 | Cell Signaling Technology | Cat#: 9172; RRID: AB_2198300 |
| Rabbit monoclonal anti-phospho-STAT1 (Tyr701) (clone 58D6) | Cell Signaling Technology | Cat#: 9167; RRID: AB_561284 |
| Rabbit polyclonal anti-MAVS | Cell Signaling Technology | Cat#: 3993; RRID: AB_823565 |
| Rabbit monoclonal anti-STING (clone D2P2F) | Cell Signaling Technology | Cat#: 13647; RRID: AB_2732796 |
| Rabbit monoclonal anti-IRF3 (clone D83B9) | Cell Signaling Technology | Cat#: 4302; RRID: AB_1904036 |
| Rabbit monoclonal anti-phospho-IRF3 (Ser396) (clone 4D4G) | Cell Signaling Technology | Cat#: 4947; RRID: AB_823547 |
| Mouse monoclonal anti-Nuclear Matrix Protein p84 (clone 5E10) | GeneTex | Cat#: GTX70220; RRID: AB_372637 |
| Mouse monoclonal anti-FLAG M2 | Sigma-Aldrich | Cat#: F3165; RRID: AB_259529 |
| Mouse monoclonal anti-HA.11 Epitope Tag (clone 16B12) | BioLegend | Cat#: 901501; RRID: AB_2565006 |
| Mouse monoclonal anti-c-Myc (Clone 9E10) | BioLegend | Cat#: 626801; RRID: AB_2235686 |
| Mouse monoclonal anti-HLA-ABC (clone W6/32) | BioLegend | Cat#: 311441; RRID: AB_2800814 |
| Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP | Thermo Fisher Scientific | Cat#: 31430; RRID: AB_228307 |
| Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP | Thermo Fisher Scientific | Cat#: 31460; RRID: AB_228341 |
| Chemicals, peptides, and recombinant proteins | ||
| Recombinant Human IFN-α2 (carrier-free) | BioLegend | Cat#: 592702 |
| Poly(I:C) (LMW)/LyoVec™ | InvivoGen | Cat#: tlrl-picwlv |
| Poly(I:C) (HMW)/LyoVec™ | InvivoGen | Cat#: tlrl-piclv |
| ADU-S100 [2′3′-c-di-AM(PS)2 (Rp,Rp)] | InvivoGen | Cat#: tlrl-nacda2r |
| TRIzol™ Reagent | Thermo Fisher Scientific (Invitrogen) | Cat#: 15596018 |
| qScript cDNA Synthesis Kit | Quantabio | Cat#: 95047 |
| iTaq Universal SYBR Green Supermix | Bio-Rad | Cat#: 1725124 |
| PEI (Polyethylenimine, branched) | Sigma-Aldrich | Cat#: 408727 |
| cOmplete™, EDTA-free Protease Inhibitor Cocktail | Sigma-Aldrich (Roche) | Cat#: 11873580001 |
| PhosSTOP™ | Sigma-Aldrich (Roche) | Cat#: 4906837001 |
| Protein G Plus/Protein A Agarose Suspension | EMD Millipore | Cat#: IP05 |
| Anti-FLAG M2 Affinity Gel | Sigma-Aldrich | Cat#: A2220 |
| Critical commercial assays | ||
| RNeasy Plus Mini Kit | QIAGEN | Cat#: 74136 |
| Immunoproteasome Activity Fluorometric Assay Kit II | UBPBio | Cat#: J4170 |
| Dual-Luciferase® Reporter Assay System | Promega | Cat#: E1980 |
| Deposited data | ||
| RNAseq data of cell lines | This paper | GEO: GSE226754 |
| RNAseq data of HPV-positive OPSCCs from Johns Hopkins University cohort | Guo et al.21 | GEO: GSE112027 |
| Experimental models: Cell lines | ||
| Human: CAL-27 | Dr. J. Silvio Gutkind (University of California, San Diego) | RRID: CVCL_1107 |
| Human: HEK293T | Dr. Dong-Er Zhang (University of California, San Diego) | RRID: CVCL_0063 |
| Mouse: DC2.4 | Dr. Dong-Er Zhang (University of California, San Diego) | RRID: CVCL_J409 |
| Mouse: MOC2 | Dr. J. Silvio Gutkind (University of California, San Diego) | RRID: CVCL_ZD33 |
| Mouse: AT-84 | Dr. Aldo Venuti (Regina Elena National Cancer Institute, Italy) | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | Strain#: 000664; RRID: IMSR_JAX:000664 |
| Mouse: C3H/HeNCrl | Charles River Laboratories | Strain Code: 025; RRID: IMSR_CRL:025 |
| Oligonucleotides | ||
| See Table S5 for qPCR primers | This paper | N/A |
| Recombinant DNA | ||
| Plasmid: pLXSN-AU1-HPV16 E5 (codon-optimized) | Dr. Frank Suprynowicz (Georgetown University Medical School) | N/A |
| Plasmid: MIP (MSCV-IRES-Puro) | Dr. Dong-Er Zhang (University of California, San Diego) | N/A |
| Plasmid: pMSCV-Blasticidin | Addgene | Plasmid: #75085; RRID: Addgene_75085 |
| Plasmid: pMSCV-Zeo | Addgene | Plasmid: #75088; RRID: Addgene_75088 |
| Plasmid: pCL-10A1 | Novus | Cat#: NBP2–29542 |
| Plasmid: pCL-Eco | Novus | Cat#: NBP2–29540 |
| Plasmid: pcDNA™3.1 (+) Mammalian Expression Vector | Thermo Fisher Scientific (Invitrogen) | Cat#: V79020 |
| Plasmid: MIP-FLAG-HPV16 E5 (codon-optimized) | This paper | N/A |
| Plasmid: pMSCV-Blasticidin-HPV16 E6 | This paper | N/A |
| Plasmid: pMSCV-Zeo-HPV16 E7 | This paper | N/A |
| Plasmid: pcDNA-FLAG-HPV16 E5 (codon-optimized) | This paper | N/A |
| Plasmid: pcDNA-HA-HPV16 E5 (codon-optimized) | This paper | N/A |
| Plasmid: pcDNA-HA-human MAVS | This paper | N/A |
| Plasmid: pcDNA-human STING-HA | This paper | N/A |
| Plasmid: pcDNA-human STING-Myc | This paper | N/A |
| Plasmid: pcDNA-HA-human IRF3 | This paper | N/A |
| Plasmid: pGL3-Basic | Promega | Cat#: E1751 |
| Plasmid: pGL3-IFN-β gene promoter | This paper | N/A |
| Plasmid: pRL-TK | Promega | Cat#: E2241 |
| Software and algorithms | ||
| GraphPad Prism 8 | GraphPad | N/A |
| ImageJ | Schneider et al.48 | https://imagej.nih.gov/ij/ |
| STAR (v2.6.1) | Dobin et al.49 | N/A |
| DESeq2 (v2_1.6.3) | Love et al.50 | N/A |
| Gene Ontology | Ashburner et al.51; Gene Ontology Consortium52; Mi et al.53 |
http://geneontology.org/ |
| Reactome | Fabregat et al.54 | https://reactome.org/ |
| KEGG | Kanehisa et al.55–57 | http://www.kegg.jp/ |
| PEAKS | Bioinformatics Solutions Inc. | N/A |
| Immune Epitope Database and Analysis Resource (IEDB) | Vita et al.58 | https://www.iedb.org |
| WebLogo 3 | Schneider et al.59; Crooks et al.60 |
https://weblogo.threeplusone.com |
| Other | ||
| MycoAlert PLUS Mycoplasma Detection Kit | Lonza | Cat#: LT07–710 |