Fig. 3. The inhibitory effect of LpK79 on IL-6 and IL-8 expression in human umbilical vein endothelial cells (HUVECs).

HUVECs were incubated with Escherichia coli LPS (1 μg/mL) with or without LpK79 (10 mg/mL) at 37°C for 24 h. IL-6 (A) and IL-8 mRNA expressions (B) were determined using qRT-PCR. IL-6 (C) and IL-8 (D) protein secretions were measured using ELISA. p<0.05 was considered statistically significant. ^a–c Different letters indicate statistical differences between groups. IL, interleukin; LPS, lipopolysaccharide; LpK79, Lactiplantibacillus plantarum K79; qRT-PCR, quantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.