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. 2023 Dec 12;300(1):105536. doi: 10.1016/j.jbc.2023.105536

Figure 1.

Figure 1

CRISPR screening reveals host factor HSP90AB1 is associated with PDCoV infection.A, schematic overview of the genome-wide CRISPR screen in LLC-PK cells. LLC-PK cells expressing Cas9 were transduced with lentiviruses encoding a pig CRISPR KO pooled sgRNA library at an MOI of 0.3. At 2 days post-transduction, cells were selected with puromycin (4 μg/ml) for 7 days and subsequently infected with PDCoV at an MOI of 0.04. PDCoV-resistant cells were expanded and harvested to isolate genomic DNA (gDNA) for Illumina sequencing. B and C, candidate genes identified by the CRISPR screening. B, data analysis was performed by using the MAGeCK method to identify enriched sgRNA. C, the corresponding genes were rank ordered by robust rank aggregation (RRA) scores. HSP90AB1, heat shock protein 90 alpha family class B1; MOI, multiplicity of infection; PDCoV, porcine deltacoronavirus; sgRNA, single-guide RNA.