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. 2023 Dec 12;300(1):105536. doi: 10.1016/j.jbc.2023.105536

Figure 2.

Figure 2

KO of HSP90AB1 suppresses PDCoV infection.A, qRT–PCR of HSP90AB1 mRNA and (B) protein levels in WT and KO cells. C, cell viability of WT and KO cells was evaluated by CCK-8 assay. D, PDCoV M mRNA levels and (E) viral titers in the cell supernatants at 24 hpi. F, N protein levels in WT and KO cells. G, IFA of PDCoV-infected WT and KO cells. Viral N protein was stained with FITC-anti-rabbit IgG (green), and nuclei were stained with DAPI (blue). Magnification = 10×, scale bar represents 100 μm. Data are shown as the mean ± SD from three independent experiments. CCK-8, Cell Counting Kit-8; DAPI, 4′,6-diamidino-2-phenylindole; HSP90AB1, heat shock protein 90 alpha family class B1; IFA, immunofluorescence assay; IgG, immunoglobulin G; PDCoV, porcine deltacoronavirus; qRT–PCR, quantitative RT–PCR.