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. 2023 Dec 12;300(1):105536. doi: 10.1016/j.jbc.2023.105536

Figure 3.

Figure 3

Overexpression of HSP90AB1 has no effect on PDCoV infection.A and B, overexpression of HSP90AB1 in the LLC-PK cells and ST cells was verified by Western blot using anti-HSP90AB1 antibody and anti-GFP antibody. C, qRT–PCR analysis of PDCoV M mRNA level in HSP90AB1-overexpressing ST cells. D, viral titer in the supernatant from PDCoV-infected HSP90AB1-overexpressing ST cells was detected by TCID50. E, Western blot detection of PDCoV N protein expression in HSP90AB1-overexpressing ST cells. The band density of the protein was quantified using ImageJ software, and the PDCoV-N to ACTB ratios were normalized to the control. Data are shown as the mean ± SD from three independent experiments. HSP90AB1, heat shock protein 90 alpha family class B1; PDCoV, porcine deltacoronavirus; qRT–PCR, quantitative RT–PCR.