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. 2023 Dec 12;300(1):105536. doi: 10.1016/j.jbc.2023.105536

Figure 8.

Figure 8

HSP90AB1 interacts with the C-tail domain of N protein.A, schematic diagram of PDCoV N protein and its truncated mutants. B and C, HEK293T cells were cotransfected with pEGFP-N1-HSP90AB1 together with (B) full-length N or its deletion mutants or with (C) NTD + LKR (its truncated mutant). The cell lysates were used for immunoprecipitation with an anti-GFP antibody, and the coprecipitated partner was detected by Western blot using an anti-HA antibody. HA, hemagglutinin; HEK293T, human embryonic kidney 293T cell line; HSP90AB1, heat shock protein 90 alpha family class B1; LKR, serine/arginine-rich linker region; NTD, N-terminal domain; PDCoV, porcine deltacoronavirus.