Table 1.
Primers used for PDCoV genes
| Target | Primer sequences (5′-3′) |
|---|---|
| N | F: tggccatggaggcccgaattGCCACCATGGCTGCACCAGTGGTCCCTACTA |
| R: ccgcggccgcggtacctcgagCTACGCTGCTGATTCCTGCTTTAT | |
| M | F: tggccatggaggcccgaattGCCACCATGTCTGACGCAGAAGAGTGG |
| R: ccgcggccgcggtacctcgagTTACATATACTTATACAGGCGAGCG | |
| E | F: tggccatggaggcccgaattGCCACCATGGTAGTCGACGACTGGGC |
| R: ccgcggccgcggtacctcgagTCAGACATAGTGAGTGTTTCTAGGA | |
| NSP5 | F: tggccatggaggcccgaattGCCACCGCAGGTATCAAAATCCTCCTGC |
| R: ccgcggccgcggtacctcgagCTGCAATGAAATTGGAGCCTGA | |
| NSP10 | F: tggccatggaggcccgaattGCCACCGCAAGTGGCACTCAAATTGAGTAC |
| R: ccgcggccgcggtacctcgagACTAGATCCACAGGTGCAGTCATAA | |
| NSP15 | F: tggccatggaggcccgaattGCCACCAACCTTGAAAACTTAGCTTACAACTG |
| R: ccgcggccgcggtacctcgagCTGTAAGATTGGGTAGCAAGTCTTG | |
| NS6 | F: tggccatggaggcccgaattGCCACCATGTGCAACTGCCATCTGCA |
| R: ccgcggccgcggtacctcgagTTAATTTAATTCATCTTCAAGAATG | |
| NS7 | F: tggccatggaggcccgaattGCCACCGAGTTCCGCTTAACTCCGC |
| R: ccgcggccgcggtacctcgagCTAGAGCCATGATGCGAGGAT | |
| ΔN-ARM | F: tggccatggaggcccgaattGCCACCACTGACGCGTCTTGGTTTCAGG |
| R: ccgcggccgcggtacctcgagCGCTGCTGATTCCTGCTTTATCTC | |
| N-ARM + NTD | F: tggccatggaggcccgaattGCCACCATGGCTGCACCAGTGGTCC |
| R: cttgGGGGTCAACTCTGAAACCTTGA | |
| CTD + C-Tail | F: tttcagagttgaccccCAAGCTCCCAAGCGGACTT |
| R: ccgcggccgcggtacctcgagCGCTGCTGATTCCTGCTTTAT | |
| ΔC-tail | F: tggccatggaggcccgaattGCCACCATGGCTGCACCAGTGGTCC |
| R: ccgcggccgcggtacctcgagCTGGTTAACGACCGTATTGAGCG | |
| NTD + LKR | F: ggccatggaggcccgaattACTGACGCGTCTTGGTTTCAG |
| R: ccgcggccgcggtacctcgagATGTTGGGTCTTACGACGTACC |
Homologous arms are represented by lowercase letters; Restriction sites are represented by bold lowercase letters.